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fluorescein conjugated streptavidin cy5  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein conjugated streptavidin cy5
    Fluorescein Conjugated Streptavidin Cy5, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescein+conjugated+avidin/Fluorescein+Avidin+DCS%2C+Cell+Sorting+Grade/pm41922652-277-5-8
    Average 95 stars, based on 414 article reviews
    fluorescein conjugated streptavidin cy5 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Immunohistochemical expression of heat shock proteins, p63 and androgen receptor in benign prostatic hyperplasia and prostatic carcinoma in the dog.
    Article Snippet: This study compared heat shock proteins Hsp60, Hsp72 and Hsp73, along with p63 and androgen receptor (AR) immunoexpression between 16 cases of benign prostatic hyperplasia (BPH) and 11 prostatic carcinomas (PCa) in dogs.. The proportion of Hsp60-positive cells was higher in PCa compared with BPH (P = 0.033), whereas the frequency and intensity of Hsp73 immunostaining did not differ significantly between the two groups.. Hsp72-immunostained nuclei formed a discontinuous layer along the basement membrane in BPH, whereas cells in this layer in PCa were negative or weakly positive.

    Article Title: Nectin-4 and p63 immunohistochemical expression in canine prostate tumourigenesis.
    Article Snippet: An avidin/biotin blocking step was performed by incubating slides for 15 min with avidin and then biotin (Avidin/Biotin Blocking Kit, Vector Laboratories) at room temperature. .. Another secondary biotinylated goat anti-mouse Ab (for p63) (1:200 dilution; Vector Laboratories) was applied and incubated for 30 min at room temperature and the slides were then treated with fluorescein-conjugated avidin (1:100 dilution in 0.1 M NaHCO3, 0.15 M NaCl buffer, pH 8.2–8.5; Vector Laboratories) for 10 min at room temperature. .. Nuclei were counterstained with 4,6-diamidino-2phenylindole (DAPI) (Vector Laboratories).

    Article Title: CD3 and CD20 Coexpression in a Case of Canine Cutaneous Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides).
    Article Snippet: A 14-year-old female spayed Dachshund was presented with generalized scaling, erythema, pruritus, poor quality of hair coat, and progressive weight loss.. Cutaneous epitheliotropic T-cell lymphoma (CETCL) was suspected.. Skin biopsies were suggestive of CETCL.

    Article Title: Comparison of Karyotypes in Two Hybridizing Passerine Species: Conserved Chromosomal Structure but Divergence in Centromeric Repeats
    Article Snippet: Post-hybridization washes were performed three times for 5 min in 50% formamide/2× SSC at 37°C and washed twice for 5 min in 2× SSC and finally for 5 min in 4× SSC/0.05% Tween 20 (Sigma Aldrich). .. Slides were first incubated for 30 min at 37°C with 4× SSC/5% blocking reagent (Roche), then the probe signal was detected by 4× SSC/5% blocking reagent mixed with fluorescein conjugated avidin (Vector laboratories) for 30 min at 37°C, followed by three washes in 4× SSC/0.05% Tween 20 for 5 min. .. Slides were then incubated with biotinylated anti-avidin (Vector laboratories) for 30 min at 37°C, followed by a second round of fluorescein conjugated avidin treatment for signal amplification.

    Avidin-Biotin Assay:

    Article Title: Immunohistochemical expression of heat shock proteins, p63 and androgen receptor in benign prostatic hyperplasia and prostatic carcinoma in the dog.
    Article Snippet: This study compared heat shock proteins Hsp60, Hsp72 and Hsp73, along with p63 and androgen receptor (AR) immunoexpression between 16 cases of benign prostatic hyperplasia (BPH) and 11 prostatic carcinomas (PCa) in dogs.. The proportion of Hsp60-positive cells was higher in PCa compared with BPH (P = 0.033), whereas the frequency and intensity of Hsp73 immunostaining did not differ significantly between the two groups.. Hsp72-immunostained nuclei formed a discontinuous layer along the basement membrane in BPH, whereas cells in this layer in PCa were negative or weakly positive.

    Article Title: Nectin-4 and p63 immunohistochemical expression in canine prostate tumourigenesis.
    Article Snippet: An avidin/biotin blocking step was performed by incubating slides for 15 min with avidin and then biotin (Avidin/Biotin Blocking Kit, Vector Laboratories) at room temperature. .. Another secondary biotinylated goat anti-mouse Ab (for p63) (1:200 dilution; Vector Laboratories) was applied and incubated for 30 min at room temperature and the slides were then treated with fluorescein-conjugated avidin (1:100 dilution in 0.1 M NaHCO3, 0.15 M NaCl buffer, pH 8.2–8.5; Vector Laboratories) for 10 min at room temperature. .. Nuclei were counterstained with 4,6-diamidino-2phenylindole (DAPI) (Vector Laboratories).

    Article Title: Full Karyotype Interphase Cell Analysis
    Article Snippet: After removing rubber cement and the coverslips, the slides were washed in 0.1× SSC at 43C for 2 min, then, when biotin or digoxigenin labels have been used, incubated in PNM blocking reagent (5% nonfat dry milk powder [NestléCarnation; Wilkes-Barre, PA], 1% Nonidet-P40 [Sigma], 1% sodium azide [Sigma], 0.1 M sodium phosphate buffer, pH 8.0) for 10 min at room temperature. .. Bound probes were detected with fluorescein-conjugated avidin (avidin DCS; Vector Laboratories Inc.; Burlingame, CA) and rhodamine-labeled anti-digoxigenin antibodies (Roche Diagnostics; Indianapolis, IN). ..

    Article Title: mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress
    Article Snippet: .. Primary antibody (Novocastra-Leica, Wetzlar, Germany) was detected by a specific biotinylated secondary antibody, followed by fluorescein-conjugated avidin (Vector Laboratories, Burlingame, CA, USA). .. Coverslips were mounted with Fluoroshield with DAPI (Sigma-Aldrich) and α -smooth muscle actin (Sigma-Aldrich) to allow visualization of the cell nuclei and cellular morphology.

    Article Title: CD3 and CD20 Coexpression in a Case of Canine Cutaneous Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides).
    Article Snippet: A 14-year-old female spayed Dachshund was presented with generalized scaling, erythema, pruritus, poor quality of hair coat, and progressive weight loss.. Cutaneous epitheliotropic T-cell lymphoma (CETCL) was suspected.. Skin biopsies were suggestive of CETCL.

    Article Title: Effect of Aging on Periodontal Inflammation, Microbial Colonization, and Disease Susceptibility
    Article Snippet: .. Primary antibody (Abcam, Cambridge, MA, USA) was detected by a specific biotinylated secondary antibody, followed by fluorescein-conjugated avidin (Vector Laboratories, Burlingame, CA, USA). .. Coverslips were mounted with Fluoroshield with DAPI (Sigma-Aldrich) to allow visualization of the cell nuclei.

    Article Title: Comparison of Karyotypes in Two Hybridizing Passerine Species: Conserved Chromosomal Structure but Divergence in Centromeric Repeats
    Article Snippet: Post-hybridization washes were performed three times for 5 min in 50% formamide/2× SSC at 37°C and washed twice for 5 min in 2× SSC and finally for 5 min in 4× SSC/0.05% Tween 20 (Sigma Aldrich). .. Slides were first incubated for 30 min at 37°C with 4× SSC/5% blocking reagent (Roche), then the probe signal was detected by 4× SSC/5% blocking reagent mixed with fluorescein conjugated avidin (Vector laboratories) for 30 min at 37°C, followed by three washes in 4× SSC/0.05% Tween 20 for 5 min. .. Slides were then incubated with biotinylated anti-avidin (Vector laboratories) for 30 min at 37°C, followed by a second round of fluorescein conjugated avidin treatment for signal amplification.

    Blocking Assay:

    Article Title: Comparison of Karyotypes in Two Hybridizing Passerine Species: Conserved Chromosomal Structure but Divergence in Centromeric Repeats
    Article Snippet: Post-hybridization washes were performed three times for 5 min in 50% formamide/2× SSC at 37°C and washed twice for 5 min in 2× SSC and finally for 5 min in 4× SSC/0.05% Tween 20 (Sigma Aldrich). .. Slides were first incubated for 30 min at 37°C with 4× SSC/5% blocking reagent (Roche), then the probe signal was detected by 4× SSC/5% blocking reagent mixed with fluorescein conjugated avidin (Vector laboratories) for 30 min at 37°C, followed by three washes in 4× SSC/0.05% Tween 20 for 5 min. .. Slides were then incubated with biotinylated anti-avidin (Vector laboratories) for 30 min at 37°C, followed by a second round of fluorescein conjugated avidin treatment for signal amplification.



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